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be similar in response to the different levels of LC. In conclusion, long-term supplementation of rooster diet with LC can have beneficial effects on SQF and testis histology. The benefits include alterations in testicular histology, reproductive hormones and testicular fatty acid profiles.Scrotal heat stress affects spermatogenesis and impairs male fertility by increasing sperm morphological abnormalities, oxidative stress and DNA fragmentation. While sperm morpho-functional changes triggered by scrotal heat stress are well described, sperm molecular alterations remain unknown. Recently, spermatozoa were described as accumulating miRNAs during the last steps of spermatogenesis and through epididymis transit, mainly by communication with small extracellular vesicles (sEVs). Herein, the aim was to investigate the impact of scrotal heat stress in miRNAs profile of sperm, as well as, seminal plasma sEVs. Six Nelore bulls (Bos indicus) were divided into two groups Control (CON; n = 3) and Scrotal Heat Stress (SHS; n = 3; scrotal heat stressed during 96 h by scrotal bags). The day that the scrotal bags were removed from SHS group was considered as D0 (Day zero). Seminal plasma sEVs were isolated from semen samples collected seven days after heat stress (D+7) to evaluate sEVs diameter, concentration,-15a, -18a, -20b, -30a-5p, -30b-5p, -30d, -30e-5p -34b, -34c, -106b, -126-5p, -146a, -191, -192, -200b, -335 and -449a). Thus, the scrotal heat stress probably impacted testicular and epididymis functions by reducing the levels of a substantial proportion of sEVs and sperm miRNAs. Our findings suggest that miR-126-5p was possibly trafficked between sEVs and sperm and provide new insights on the mechanism by which sperm acquire miRNAs in the last stages of spermatogenesis and sperm maturation in cattle.Stress has deleterious impact on semen quality and fertility of roosters. This study was investigated to know whether dietary supplementation of organic selenium (oSe) could improve semen quality and fertility of male broiler breeder under dexamethasone (Dexa) induced stress. Forty broiler breeder roosters (64 week of age) were randomly allotted to four groups (10 roosters/group) and fed a standard diet supplemented with different levels of oSe during 10 successive weeks of the experimental period. To induce stress, the birds received injections of 2 mg/kg BW of Dexa during weeks 5 and 6 of the experiment, in one-day-intervals manner. The roosters were not treated with Dexa and oSe (negative control; NC), or treated with Dexa and different levels of oSe including 0 (positive control; PC), 0.30 (Se30+Dexa) or 0.45 (Se45+Dexa) mg/kg diet. Body weight was measured weekly and semen quality parameters and fertility were evaluated every two weeks. click here Except for seminal volume and total sperm production which was not affected by the treatment, body weight, semen quality parameters, total antioxidant capacity (TAC) and malondialdehyde (MDA) in seminal plasma were influenced by interactive effect of treatment and time (P less then 0.05). Dexamethasone injection adversely affected semen quality parameters (semen concentration, motility and plasma membrane integrity) in PC group compared to NC group (P less then 0.05); however, dietary supplementation of oSe ameliorated these negative impacts in Dexa-treated roosters (P less then 0.05). Fertility was also improved by dietary supplementation of oSe compared to control groups (P less then 0.05). These results indicate that although induction of stress have negative effects on rooster semen quality parameters, dietary inclusion of oSe may exert beneficial impact on mitigating the harmful effects of stress on semen quality and fertility rate of broiler breeder roosters.

Evaluation of long-term efficacy and safety of various anti-hyperglycaemic agents (AHA) for glycaemic control in NODAT, in stable kidney transplant recipients (KTRs) during 1-year outpatient follow-up.

We collected FPG, PPG, HbA1c, serum creatinine, eGFR, blood tacrolimus level, hypoglycaemia and body weight values from an existing database of KTRs diagnosed to have NODAT. Those newly initiated on AHA over 3 months post-transplant; received standard triple immunosuppressive therapy; and followed up for 1-year after referral, were included.

In ninety-five patients’ (Male=65), mean decrease at 1-year from baseline in FPG (185.01±62.11mg/dL), PPG (293.21±85.23mg/dL) and HbA1c (8.48±1.08%) was 67.09, 126.11 and 1.4 respectively (p<0.0001). At 1-year, mean HbA1c was 7.08±0.38%, ninety-one patients achieving HbA1c≤7.5%. Fifty-two patients received oral combination therapy based on linagliptin/metformin/repaglinide/gliclazide, 19 received insulin-based regimen, and 24 received linagliptin monotharapey. Thirty patients reported hypoglycaemia (10 with gliclazide and 15 with insulin) and fifty patients gained body-weight at 1-year. Mean serum creatinine and eGFR significantly improved by 0.29 and 15.77 from baseline of 1.56±0.62mg/dL and 53.95±16.10mL/min/1.73m

respectively.

Significant proportion of NODAT patients achieved long-term glycemic control with improved renal function. Combination therapy was needed in most within 1-year. Linagliptin monotherapy was effective, without producing hypoglycaemia or weight gain.

Significant proportion of NODAT patients achieved long-term glycemic control with improved renal function. Combination therapy was needed in most within 1-year. Linagliptin monotherapy was effective, without producing hypoglycaemia or weight gain.

to understand how undergraduate students with history of suicidal thoughts retrieve information on suicide method.

This is a cross-sectional descriptive study using custom self-rated questionnaires consisting of demographic data, history of previous suicidal thoughts or attempts, current suicidal risks via 8q questionnaires, known methods of suicide and history of suicidal attempt or planning and information on sources of suicide method. Participants were Thai undergraduate students of Mae Fah Luang University who were visiting psychiatric outpatient clinic of the university hospital and had history of suicidal thoughts.

Out of 39 participants, 25 participants reported intentionally searching for information on suicide methods while 31 participants reported unintentionally acquiring information on suicide methods. 19 participants reported that they both intentionally searching for the information and unintentionally acquiring it. The internet was used by all 25 participants who intentionally searched for information on suicide methods, with the second most common way was retrieving information from other suicidal attempters (reported by 11 participants).